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3D-Tissue Culture Reveals New Targets for Cancer Therapy3D-Tissue Culture Reveals New Targets for Cancer Therapy 3D-Tissue Culture Reveals New Targets for Cancer Therapy (from National Cancer Institute) Over the past few years, research has shown clearly that tumor cells behave differently when allowed to grow...

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Why culture cells in 3 dimensi...Why culture cells in 3 dimensi... Why culture cells in 3 dimensions? http://www.3dcellculture.com/Big_Picture_3D_vs_2D_Cell_Culture

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3D-Tissue Culture Reveals New Targets for Cancer Therapy

Posted by admin | Posted in 3D Cell Culture | Posted on 20-09-2010

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3D-Tissue Culture Reveals New Targets for Cancer Therapy (from National Cancer Institute)

Over the past few years, research has shown clearly that tumor cells behave differently when allowed to grow in three dimensions rather than simply in two dimensions on the surface of a culture dish.  Indeed, now that cancer biologists have developed several methods for reproducibly growing malignant cells in three dimensions, they are finding new approaches to therapy that were not apparent from older, two-dimensional studies.

Two papers published recently in the journal Cancer Researchhighlight the value of mastering three-dimensional cell culture techniques.  In one paper, Catherine Park and her colleagues at the Lawrence Berkeley National Laboratory and the University of California, San Francisco, demonstrate that a cell adhesion molecule known as α5β1integrin enables breast cancer cells to survive radiation therapy.  More importantly, they then showed a small peptide can prevent α5β1-integrin from binding to the protein fibronectin, which in turn heightens the cell killing effects of radiation.

Integrins are a family of molecules that play a critical role in how cells bind to and interact with one another.  In this study, Dr. Park and her collaborators were able to show that malignant cells growing in three-dimensional culture greatly overproduced α5β1 integrin after radiation treatment.  At the same time, using gene expression data from 295 breast cancer patients, the investigators found that elevated expression of the α5β1 integrin gene was associated with poor survival, suggesting that inhibitors of α5β1 integrin could prove useful in boosting the efficacy of radiation therapy in human breast cancer patients.

In a second, unrelated paper, Anil Rustgi and his colleagues from the University of Pennsylvania describe a series of experiments using three-dimensional cell culture techniques that show that a different cell adhesion molecule, known as periostin, boosts the invasiveness of esophageal tumors.  With this information in hand, the investigators then examined gene expression data from human esophageal cancer patients and found that primary invasive esophageal cancer has a distinct profile that differs from adjacent normal human esophageal tissue. With further study, these findings could be useful both in the search for new therapeutic targets and for developing a prognostic indicator for esophageal cancer.

The study on breast cancer sensitivity to radiation therapy, which was supported in part by the National Cancer Institute, is detailed in a paper titled, “Breast Cancer Cells in Three-dimensional Culture Display an Enhanced Radioresponse after Coordinate Targeting of Integrin α5β1 and Fibronectin.”  An abstract of this paper is available at the journal’s Web site.
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The study on tumor invasion, which was supported in part by the National Cancer Institute,  is detailed in a paper titled, “Periostin, a Cell Adhesion Molecule, Facilitates Invasion in the Tumor Microenvironment and Annotates a Novel Tumor-Invasive Signature in Esophageal Cancer.”  An abstract of this paper is available at the journal’s Web site.
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Researchers on path toward artificial ovary (The Boston Globe)

Posted by admin | Posted in 3D Cell Culture | Posted on 20-09-2010

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Researchers on path toward artificial ovary (The Boston Globe)

A cancer diagnosis for a young woman can involve more than just the ordeal of undergoing treatment with drugs or radiation — the therapies may also ruin her dream of having children.

But as survival rates for young cancer patients have increased, scientists have increasingly focused on creating new options, including an artificial human ovary that could allow women to more easily preserve their fertility.

In a step that combines biology and engineering, researchers at Women & Infants Hospital of Rhode Island and Brown University have built a honeycomb structure of cells that mimic the ovary, allowing an egg to mature outside the body. Their work was published last month in the Journal of Assisted Reproduction and Genetics. continue reading…

Technical Advantages of the 3D Petri Dish

Posted by admin | Posted in 3D Cell Culture | Posted on 14-09-2010

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Technical Advantages of the 3D Petri DishTM

  • Promotes natural cell-to-cell interactions.
  • Microtissues are easily and quickly formed.
  • Microtissues size is uniform and easily controlled.
  • Mixed microtissues with two or more cell types are easily produced.
  • Microtissue shape is easily controlled.
  • Microtissues are easily observed and quantified by microscopy.
  • Microtissue growth and metabolism are easily monitored.
  • Microtissues can be grown from singe cells (clones).
  • Large numbers of microtissues are easily produced.
  • Microtissues are easily harvested for biochemical analyses.
  • Microtissue production can be automated.